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Figure 1


Figure 1 (A) Dihydroethidium (DHE) was used to visualize and quantify the production of superoxide in situ. Wild-type (WT) and harlequin (Hq) hearts demonstrated a significantly higher level of superoxide production after aortic banding; this increase was attenuated by administration of antioxidant EUK-8. (B) Left ventricular myocardial sections were subjected to immunostaining with 8-OHdG, a marker of oxidative DNA damage, which was increased in nontreated mice after aortic banding; however, EUK-8–treated mice had similar levels of 8-OHdG staining as sham-operated mice.





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