Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 6 Platelet-derived growth factor receptor (PDGFR)-ß inhibition resulted in impaired vascular maturation and enhanced capillary density in the healing infarct. (A) CD31 immunohistochemistry identified the infarct vasculature in immunoglobulin IgG-treated mice (magnification 200x). (B) Numerous coated vessels were noted in the infarct after 7 days of reperfusion (arrows). (C to F) The PDGFR-ß inhibition with the neutralizing antibody APB5 resulted in impaired vascular maturation and in formation of dilated, disorganized, irregularly-shaped vascular structures (arrows). (C, magnification 200x; D to F, magnification 400x). (G to I) Quantitative analysis of total microvascular density (G), density of uncoated vessels (H), and density of coated vessels (I) in the infarct. The PDGFR-ß inhibition significantly increased microvascular density (**p < 0.01 vs. IgG-treated animals) (G) and the number of uncoated vessels (**p < 0.01 vs. IgG-injected mice) (H), but decreased the density of coated vessels (I) in the infarcted area (*p < 0.05 vs. IgG-treated mice). In contrast, PDGFR- inhibition did not affect vascular maturation. Other abbreviations as in Figure 5.
|
