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Figure 1 The microvascular network in reperfused mouse infarcts is identified by CD31 immunohistochemistry. (A) CD31 staining of control heart labels the myocardial vasculature. (B) Serial section with omission of the primary antibody serves as a negative control. (C) After 72 h of reperfusion, infarct granulation tissue contains many CD31-positive endothelial cells (arrowheads). (D) After 7 days of reperfusion, the infarcted area contains relatively few capillaries (arrows) and a significant number of coated vessels (arrowheads). Counterstained with eosin (magnification 200x).
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