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Figure 5 Time-lapse analysis of intracellular mitochondrial matrix calcium in neonatal rat cardiac myocytes. (A) Representative sequential images of rhod-2 (Molecular Probes, Eugene, Oregon) fluorescence in each group. H = cells exposed to 50 µmol/l H2O2 for 1 h; SFA = cells pretreated with 100 µmol/l serofendic acid for 30 min followed by 50 µmol/l H2O2 for 1 h. Scale bars at 0 min frames: 20 µm. (B) Time course of rhod-2 fluorescence of 25 cells randomly selected in each group. Similar results were obtained in three independent experiments. (C) Mean fluorescence intensity from 25 cells randomly and prospectively selected in each group. *p < 0.05 versus H at the end of the experimental period. Other abbreviations as in Figure 1. Please see the for an accompanying video to this figure.
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