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Figure 1 Characterization and outgrowth of isolated porcine endothelial progenitor cells (EPCs). (A) Mononuclear cells were plated on culture dishes in the presence (1 µg/ml or 100 µg/ml, respectively) or absence of integrin-binding cyclic Arg-Gly-Asp peptide (cRGD) coating. Coating with cRGD provoked a significant dose-dependent increase of spindle-shaped cells compared with uncoated control experiments. Representative images from four isolations are shown, magnification 100x. (B) Flow cytometry analysis of adherent cells at day 7 of culture. Unstained cells served as a control condition. Analysis was performed by manual gating and fluorescence channel (FL)-1/FL-2 dot plot quadrant statistic. 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine-labeled Ac-low-density lipoprotein/lectin double-positive cells in the upper right region were judged as EPCs. FITC = fluoresceinisothiocyanat.
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