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Figure 2 Immunohistochemistry of the human mitral degenerative valves and calcified tricuspid and bicuspid aortic valves for non-collagenous bone matrix synthesis. Control valve, degenerative mitral valve (arrow points to hypertrophic chondrocytes), calcified aortic valves (arrow points to positive stain), and bicuspid aortic valve (arrow points to positive stain) (magnification 25x). Insert within each photo is a high-power magnification to demonstrate cellular staining (magnification 40x). (A) Bone sialoprotein stain. (B) Osteopontin stain. (C) Osteocalcin stain.
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