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Figure 6 Midostaurin increases NO production in the aorta and serum levels of nitrite/nitrate in SHR and WKY. SHR and WKY were treated orally with vehicle or midostaurin (+Mido, 75 mg/kg/day) for 5 days. Bioactive NO in SHR aorta was assessed by electron paramagnetic resonance spin trapping technique using colloid Fe(DETC)2. Serum nitrite/nitrate was measured with an NO analyzer. (A) Original spectra. Arrows indicate the typical triplet caused by NO-Fe(DETC)2. (B) Quantification of NO-trapping experiments from the aortas of 12 animals normalized to aortic area and incubation time. (C) Serum levels of nitrite/nitrate in 36 SHR and 24 WKY. Columns represent mean ± SEM (***p < 0.001, tested by ANOVA followed by Fisher protected least-significant-difference test). Abbreviations as in Figure 1.
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