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Figure 7 Effect of different platelet inhibitors on PAC-1 binding to activated platelets. These experiments were performed as described in the caption for Figure 6, with the only difference that the P2Y1 inhibitor, MRS2179, or the putative Ca2+ channel blocker, lanthanum chloride, was also added to platelets after activation by adenosine diphosphate and epinephrine. The upper panel shows the mean and SEM of the median fluorescence of 10,000 platelets measured 30 min after addition of AR-C69931MX (final concentration: 100 nmol/l), MRS2179 (final concentration: 100 µmol/l), or lanthanum chloride (final concentration: 1 mmol/l) as compared to control in which only buffer was added (n = 8). The lower panels show the actual flow cytometric results of one representative experiment. Solid lines represent the results in the presence of the inhibitor shown in each panel, while dotted lines represent the results in the absence of inhibition.
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