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Figure 1 Three-dimensional projection imaging of platelet thrombi. A piezo-electric motor (a) moved the objective lens at a constant speed of 0.4 µmol/l/s to provide scanning images of the platelet thrombi (a’). The sum of the confocal images in a bottom to top stack (z-axis) was projected on planes at 10° intervals relative to the x axis to obtain the three-dimensional projection images shown on the right, including projections at 0 degrees (top view; A), 60° (B), and 90° (front view; C). The maximum height (h) of the platelet thrombi was calculated from the front view projection, as shown in C.
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