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Figure 3 (A) The thrombospondin (TSP)-4 mutation. Presence of mRNA and protein in endothelial cells, and abnormal calcium binding. (B) Effect of (P387)TSP-4 on proliferation of endothelial cells (EC). (C) Effect of TSP-4 variants and their fragments on adhesion of human umbilical vein endothelial cells. Cells were plated on plastic preincubated with purified TSP-4 variants (50,000 cells/well of 24-well plate), and photographs were taken after 24 h of culture (with permission from Stenina et al. [14]. mut = mutant(P387); vWF = von Willebrand factor; wt = wild type.
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