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Figure 5 Effect of hypoxia-inducible hHO-1 treatment on protecting mesenchymal stem cell (MSC) against hypoxia/reoxygen damage in vitro. (A) Hypoxia-induced hHO-1 overexpression reduces MSC apoptosis in vitro by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate in situ nick end labeling assay. After hypoxia/reoxygen/hypoxia treatment, most of MSCHO-1 expressed human HO-1 in immunostaining whereas human HO-1 expression was low in MSCLacZ or MSCs. The increase in human HO-1 expression in MSCHO-1 was accompanied by reduction in the cell apoptosis. (B) A down-regulation in a proapoptotic gene Bax level in the cell lysate of MSCHO-1 was confirmed by Western blot in comparison with MSCLacZ and MSCs after hypoxia/reoxygen/hypoxia in vitro.
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