Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 4 Hypoxia-induced overexpression of reporter gene in MSCHO-1 and MSCLacZ. (A) Immunostaining for human HO-1 shows that 24 h of hypoxia increased the expression of hHO-1 expression compared with normoxia (1 and 2). Beta-gal staining in vitro demonstrated that higher level expression of LacZ in hypoxia treated MSCLacZ compared with normoxia (3 and 4). Almost 80% transfected MSCs were positively stained for LacZ after hypoxic treatment. (B) Western blot demonstrated that the abundances of hHO-1 fusion protein were lower in MSCHO-1 at normoxia in comparison with hypoxia with 6xHis antibody for detection. (C) The hHO-1 fusion protein level of MSCHO-1 in hypoxia was 5.18-fold more abundant than in normoxia (mean ± SEM; p < 0.001; n = 3).
|
