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Figure 3 (A) Staining for cyclooxygenase (COX)-1 (red) indicated expression in the endothelium of quiescent arteries in the control hind limb. In proliferating collateral arteries, transmigrated CD11b-positive leukocytes (green) reveal a strong positive stain and serve as additional sources of COX-1. (B) Immunohistological staining for vascular smooth muscle cells (green) and accumulating monocytes/macrophages (red) in the surrounding tissue of the proliferating collateral arteries (first two columns). Proliferating cells (third column) stained for the Ki-67 antigen (red) are mainly localized in the media of the growing vessels. Vascular smooth muscle cells are stained green and nuclei appear blue. Aspirin treatment significantly reduced leukocyte migration and proliferation; clopidogrel-treated animals showed comparable results as saline-treated animals.
