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Figure 10 (A) High-power image of microvessels identified with the monoclonal endothelial cell marker CD34 linked to a blue chromogen. (B) High-power image from the plaque shoulder region showing CD34-positive microvessels in blue contrasting sharply with CD68/CD3-positive inflammatory cells linked to a red chromogen. (C) Microvessels at the tunica media (purple chromogen) contrasting with smooth muscle cells linked to a brown chromogen using alpha-actin marker. (D) Corresponding high power from C, showing disarray of smooth muscle cells in brown and microvessels in purple. Reproduced with permission from Moreno PR, et al. Circulation 2004;110:2032–8.
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