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Figure 2 (A) Representative reverse transcriptase-polymerase chain reaction (RT-PCR) showing VEGF165 and VEGF receptors (flt-1 and flk-1) messenger ribonucleic acid (mRNA) levels in the myocardial samples from control human hearts, non-diabetic and diabetic patients with CHD. 1 to 3 = control specimens; 4 to 6 = non-diabetic patients with chronic CHD; 7 to 9 = diabetic patients with chronic CHD. (B) Real-time RT-PCR quantification of myocardial VEGF, flt-1 and flk-1 mRNA. Data are the ratio values of VEGF/B-Actin, Flt-1/B-Actin, and Flk-1/B-Actin mRNA (arbitrary units). *Statistical difference from control specimens; **statistical difference from non-diabetic patients. bp = base pair; other abbreviations as in Figure 1.
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