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Figure 6 Plasma-dependent activation of nuclear factor-kB (NF-kB) in monocytes. Monocytes from healthy volunteers were evaluated for NF-kB activation after they were cultured for 20 h in medium supplemented with either 40% serum from 10 unstable angina (UA) patients with the highest oxidized low-density lipoprotein (ox-LDL) plasma concentrations (range from 41 to 62 µg/ml) or 40% serum from 10 control subjects with the lowest ox-LDL levels (range from 4 to 7 µg/ml). Monocytes were also incubated with the corresponding lipoprotein-depleted serum (LPDS). In some experiments, anti-lectin-like ox-LDL receptor-1 (LOX-1) monoclonal antibody (mAb) (20µg/ml) or control mouse immunoglobulin G (50 µg/ml) was also added to the cell culture. Results are means ± SD of experiments performed in triplicate on six separate occasions. *p < 0.001 vs. serum from control subjects (C);  p < 0.001 vs. serum UA + anti-LOX-1 mAb;  p < 0.001 vs. LPDS UA;  p < 0.01 vs. LPDS C.
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