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Figure 5 Oxidized low-density lipoprotein (Ox-LDL)-dependent activation of nuclear factor-kB (NF-kB) in monocytes and lymphocytes. The purified monocytes and lymphocytes (3 x 105/ml, 200 µl/well) were cultured in 96-well trays for 20 h at 37°C with increasing amounts of Ox-LDL in the presence of control mouse immunoglobulin G (IgG) (50 µg/ml) or anti-LOX-1 monoclonal antibody (mAb) (20 µg/ml). The NF-kB was measured in cellular extract as described in the Methods section. Results are means ± SD of experiments performed in triplicate on six separate occasions. *p < 0.001 vs. control value (ox-LDL = 0 mg/ml);  p < 0.001 vs. anti-LOX-1 mAb and lymphocytes. White bars = Ox-LDL + monocytes + IgG; black bars = Ox-LDL + monocytes + anti-LOX-1 mAb; ruled bars = Ox-LDL + lymphocytes + IgG.
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