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Figure 2 Western blot for tissue factor isoforms from explanted dilated cardiomyopathy hearts. Extracts from explanted dilated cardiomyopathy hearts were immunoprecipitated with anti-human tissue factor (TF) antibodies directed against the TF extracellular domain. Western blots showed both, full-length TF and alternatively spliced human tissue factor (asHTF) (lane 1), detected by a polyclonal anti-TF antibody (pAb-sTF) directed against the extracellular domain of the TF protein. Rehybridization of the membrane with a polyclonal antibody (asHTF), directed against the last 29 amino acids of the unique asHTF end yielded a single protein band at approximately 30 kDa, whereas full length TF was not detected (lane 2).
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