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Figure 3 Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) inducibility by oxidized low-density lipoprotein (oxLDL) and lysophosphatidylcholine (LPC). (A) Oxidized low-density lipoprotein was incubated with peripheral blood mononuclear cells for the indicated time points. Ribonucleic acid was extracted as described in the Methods section, and real-time reverse-transcription polymerase chain reaction was used to investigate the inducibility of messenger ribonucleic acid for TRAIL. Results are expressed after normalization with beta-actin mRNA as an x-fold increase from baseline. (B) For detection of the TRAIL protein, OxLDL (50 µg/ml) or LPC (at the indicated concentrations) were incubated with peripheral blood mononuclear cells, after which cell lysates were subjected to Western blotting with anti-TRAIL antibodies, compared with expression of beta-tubulin, and subjected to densitometric analysis.
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