Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 4 The effect of C-type natriuretic peptide (CNP) on messenger ribonucleic acid (mRNA) expression associated with cardiac remodeling after myocardial infarction (MI). (A) Typical autoradiograms of Northern blot analysis of mRNA levels in right ventricle (RV) and noninfarcted left ventricle (LV) for collagen type I, collagen type III, fibronectin, transforming growth factor-(TGF)-ß-1, atrial natriuretic peptide (ANP), ß- and  -myosin heavy chain (MHC), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) at the 18th day after MI. (B) Quantitative analyses of the abundance of each gene in the RV and noninfarcted LV at the 18th day after MI (n = 10 in each group). In individual samples, each mRNA value was corrected for the GAPDH mRNA value. Levels in sham-operated rats were arbitrarily assigned a value of 1.0. Values are mean ± SEM. **p < 0.01, *p < 0.05 compared with the sham-operated group; #p < 0.05 compared with the MI+vehicle group by analysis of variance and Bonferroni multiple-comparison t test. Open bars = sham; striped bars = MI+vehicle; solid bars = MI+CNP.
|
