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Figure 5 Coronary microvascular responses to acetylcholine before and after coronary ischemia/reperfusion (I/R) injury in dogs in vivo. Under control conditions, I/R significantly impaired coronary vasodilator response to acetylcholine (a), whereas hydroxyfasudil completely preserved the responses in the absence (c) or presence of L-NMMA (d) compared with that in the presence of L-NMMA alone (b). Number of vessels per animals used was 19/7 under control conditions (before I/R: y = –0.3x + 35.9, r = 0.85; after I/R: y = –0.2x + 18.1, r = 0.80), 13/4 for L-NMMA alone (before I/R: y = –0.2x + 35.1, r = 0.76; after I/R: y = –0.2x + 12.2, r = 0.88), 14/7 for hydroxyfasudil (before I/R: y = –0.2x + 27.9, r = 0.73; after I/R: y = –0.2x + 27.4, r = 0.80), and 16/7 for hydroxyfasudil plus L-NMMA (before I/R: y = –0.2x + 31.8, r = 0.83; after I/R: y = –0.2x + 19.2, r = 0.86). **p < 0.01. Open circles = before I/R; solid circles = after I/R.
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