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Figure 1 (A) An immunofluorescent photomicrograph showing CD144-expression in human coronary artery endothelial cells (HCAEC). The green signal indicates fluorescein isothiocyanate (FITC)-CD144 while the blue signal indicates the nucleus (n = 4). (B) A histogram analysis of CD144-expression in HCAEC detected by FACScalibur. The thin line represents control immunoglobulin G (IgG) and the thick line represents CD144 (n = 5). (C to H) Histogram analyses of CD144-expression in microparticles derived from a variety of cells involved in atherosclerosis detected using FACScalibur. (C) HCAEC; (D) HAoSMC (human aorta smooth muscle cell); (E) platelets; (F) lymphocytes; (G) monocytes; (H) macrophages. The thin lines represent control IgG and the thick lines represent CD144 (n = 5). (I to J) Histogram analyses of CD31-expression in microparticles derived from HCAEC and platelets. (K) Representative dot-plot graphs of microparticles expressing CD31-CD42b derived from human platelets. The lower right section of graph shows CD31-positive and CD42b-negative microparticles derived from isolated human platelets.
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