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Figure 6 (A) Line plots showing concentration response to acetylcholine in arteries incubated with and without lysophosphatidylcholine (LPC) (50 µM) for 30 to 45 min under different treatment conditions—control, reconstituted high-density lipoprotein (HDL) using apo A-IMilano (apo A-IM/DMPC), apo A-Iwild-type (apo A-IWT/DMPC), plasma-derived HDL, free apo A-IMilano without DMPC (apo A-IM), and DMPC alone. A concentration of 1 mg/ml based on protein was used for all preparations. The concentration of DMPC was based on a protein:phospholipid 1:3 molar ratio. (B) Bar graphs showing maximal dilator response to acetylcholine (top panel) and sodium nitroprusside (bottom panel) in rabbit carotid arteries under different treatment conditions. Values (mean ± SEM) are percent change from a preconstricted diameter of 1.9 ± 0.1 mm. p values are based on comparisons before and after incubation with LPC. Open bars = –LPC (50 µM); solid bars = +LPC (50 µM). *p < 0.005 vs. control + LPC.
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