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Figure 4 Western blot analysis demonstrating the activation of mitogen-activated protein kinases (MAPKs) (ERK-1/2, JNK-1/2, p38) and protein kinase C (PKC)- translocation to the particulate fraction. Each lane was loaded with equal amounts of protein. Polyclonal antibodies were used to detect bands. The upper panel of each figure is a representative blot, whereas the lower panels depict grouped densitometric data (mean ± SEM) from three experiments. For MAPKs, we used the ratio of phosphorylated MAPK/non-phosphorylated MAPK. (A) The upper panel shows a representative blot of the amount of PKC- translocation to the particulate fraction, whereas the lower panel demonstrates the quantitative densitometric data of PKC- translocation to the particulate fraction. (B to D) The upper panels show a representative blot of the activated form of each MAPK subtype, whereas the lower panel depicts the quantification of densitometric data. (B) phospho-ERK, (C) phospho-JNK1/2, (D) phospho-p38. CHE = chelerythrine; FGF = fibroblast growth factor transgenic littermates; IR = ischemia and reperfusion; LA = lavendustin A; PKC = protein kinase C; WT = wild-type littermates.
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