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Figure 1 Transplant myoblasts. (A) Phase contrast images. (B) Desmin-labeled cells. (C) Hoechst 33342-labeled nuclei. Transplant rat myoblasts (bottom panel) were stained with desmin and hoechst to verify myoblast percentage. Desmin staining indicates that cultured cell populations, which were subsequently injected into the myocardium, contained >95% myoblasts. One contaminating fibroblast is indicated by the arrows. Control rat fibroblasts and rat myoblasts from the L6 cell line were stained with desmin and hoechst as negative and positive controls, respectively.
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