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Figure 3 Expression of selected signaling molecules after short-term induced expression of cTnT-Q92 in bigenic mice. (A) Immunoblots showing levels of the major components of PI3K pathway: each lane represents one experimental group, as labeled. Bigenic mice were injected with either a placebo or mifepristone (Mife On) at 1,000 µg/kg/day for 16 days. Mife Off indicates bigenic mice after withdrawal of mifepristone for 16 days. (B) Immunoblots showing expression levels of selected phosphorylated PKC isoforms; the bottom panel shows expression levels of sarcomeric actin, used as a control for loading condition. Groups are as indicated for panel A. (C, D, and E) Quantitative levels of PTEN, p-PKC-Z -T538, and p-PKD-S744-748. The F and p values are shown for the entire groups. All p values were <0.05 when levels of PTEN, p-PKC-Z-T538, and p-PKD-S744-748 were compared with their corresponding levels in other experimental groups (ANOVA).
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