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Figure 2 In vivo iron oxide injection experiments. (A) As shown in this procedure flow map, a solution of iron oxide particles and tissue dye (either methylene blue or India ink) was injected into the myocardium of normal animals at two to three discrete LV locations (left). Because the iron oxide particles are visible to MRI (middle), these injections served as "targets" (shown by an arrow in B) to test the registration strategy. The animals underwent MRI, and manual segmentation was performed to delineate the endoluminal surface of the aorta, the endocardial border of the LV, and the locations of the iron oxide injections (shown in C; each group of three blue dots represents an injection site). During the subsequent electroanatomic mapping procedure, these 3D datasets were registered with the MEAM system. Because the iron oxide injections do not leave any electrophysiologic signature, the locations of the ablation lesions were entirely based upon the registered MR images.
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