Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 2 Confocal microscopy (Zeiss LSM 510 UV, 100x oil immersion objective) of a representative sample illustrates that pre-exercise adherent cells were positive for the uptake of DiI-labeled acetylated low-density lipoprotein (red) (a) and the binding of FITC-Ulex-Lectin (green) (b). Visualization of nuclei with DAPI (blue) (c) and the overlay of all three fluorescence images (d) demonstrate that all adherent cells fulfill cultured angiogenic cell criteria (acLDL+/Ulex-Lectin+). Respective post-exercise cultured angiogenic cells are shown in the lower panels (e to h). Bar = 25 µm.
|
