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Figure 2 Mitochondrial stress in ischemic and reperfused hearts. (A) Representative electron microscopy sections taken from control (CON), 20', 58', and reperfusion (REP) left ventricular samples showing mitochondrial swelling (scale bars = 0.2 µm). We examined at least 10 fields/samples, counted at least two to three mitochondria/fields, and compared their median size to the scale bar indicated. In the CON samples, we did not find enlarged mitochondria (2 x the scale bar). (B) Biochemical determination of cytochrome C oxidase activity, ATP content, and malondialdehyde (MDA) levels on ventricular samples. For cytochrome C oxidase, *p < 0.01 vs. CON; for ATP levels, *p < 0.01 for ischemia and REP vs. CON and #p < 0.05 for REP vs. ischemia; for MDA, *p < 0.01 vs. CON. Abbreviations as defined in Figure 1.
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