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Figure 1 Representative scatter plots of flow cytometric settings for detecting platelet-monocyte and platelet-neutrophil conjugates using anti-CD14-PC5 and anti-CD42a-FITC. (Left panel) The leukocyte populations are distinguished by their granularities (SS = side scale) and CD14 densities (anti-CD14-PC5). The cell population with higher CD14-PC5 intensity but lower granularity represents monocytes (gate B), whereas the population with lower CD14 intensity but higher granularity represents neutrophils (gate I). Monocytes (gate B) and neutrophils (gate I) are further analyzed by CD42a-FITC (platelet marker) versus CD14-PC5 (middle and right panels, respectively). The particles positive for both anti-CD42a-FITC and anti-CD14-PC5 in region "2" of the scatter plot are identified as platelet-monocyte conjugates (middle panel) and platelet-neutrophil conjugates (right panel).
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