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Figure 4 (A, panels a to d) Immunohistochemical staining for von Willebrand factor in the triceps muscle of the left calf revealed the presence of numerous vessels. Vessels were larger and more numerous in the NIH3T3 + hHGF + TK group (panels c and d) than in the saline (panel a) and NIH3T3 groups (panel b). Scale bars = 100 µm. (B) The number of vessels was determined by observation of 20 random fields from 10 mice (2 fields per mouse; *p < 0.01). (C) Distribution of the minimum diameters of the von Willebrand factor-positive vessels (n = 25; *p < 0.0001). Abbreviations as in Figure 1.
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