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Figure 5 Beta-adrenergic receptor kinase 1 (ARK1) and G-protein–coupled receptor kinase (GRK) 5 protein content. (A) Immunoblotting showed that the beta-ARK1 content in the particulate fraction was increased in noninfarcted viable myocardium of the untreated myocardial infarction (MI) animals (MI/control [C]) as compared with shams. Rats with MI treated with candesartan (MI/angiotensin II type 1 receptor blocker [ARB]) exhibited a reduction in beta-ARK1 protein level of noninfarcted viable myocardium, as compared with MI/C rats. (B) Immunoblotting showed that there was no significant difference in beta-ARK1 content in the supernatant fraction among the groups. (C) Representative immunoblots for GRK5 are shown. The GRK5 protein content in the particulate fraction did not change among the three groups. *p < 0.001 MI/C vs. sham;  p < 0.01 MI/ARB vs. MI/C; n = 6 in each group.
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