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Figure 1 (A) Typical autoradiopraph of light-dependent in vitro phosphorylation of rhodopsin (32P-ROS) by atrial cytosolic (CYT) and membranous (MEM) GRK obtained from a child (3-year-old male; NYHA class 0) and an elderly patient (60-year-old male; NYHA class I). (B) Bar graph showing cytosolic and membranous GRK activity in right atrial appendages obtained from 10 children (5 males and 5 females; mean age 6 ± 2 years [range 3 months to 20 years]; NYHA class 0.9 ± 0.3) and eight elderly patients (4 males and 4 females; mean age 68 ± 3 years [range 59 to 80]; NYHA class 1.9 ± 0.4). Activities were calculated as 32P incorporated in rhodopsin in cpm x 103 per mg protein. Data are given as the mean value ± SEM.
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