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Figure 2 Mouse holder. The line source is a circular hole machined into the base of the mouse holder (above the mounting hole) capped by a plastic screw and is colinear to the axis of rotation. After the line source is imaged, the entire assembly is translated vertically to position the mouse in front of the pinhole. As shown in the figure, the mouse’s thorax is within the field of view but the line source is displaced from the field of view when the mouse is imaged. Most of the mouse’s weight is supported by the bottom of the tube and not by the forepaw restraints.
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