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Figure 7 Immunohistochemical analysis of Cypher/ZASP expression. Immunohistochemical analysis of C2C12 cells transfected with the pcDNA3.1/NT-GFP-TOPO vector (A to C), or with constructs expressing wild type Cypher/ZASP-1-GFP (D-F) or D117N-Cypher/ZASP-1-GFP (G to I). Actin staining (red) is shown in the left panels, GFP (Cypher/ZASP ) staining (green) is shown in the middle panels, while the right panels are the overlay of the ZASP, actin and DAPI (nuclei) images. All images were obtained using 40x magnification.
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