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Figure 4 Activity of NF-kB in heart tissue after encephalomyocarditis virus (EMCV)-induced myocarditis inoculation. (A) Representative autoradiogram of electrophoretic mobility shift assay (EMSA) experiment. A supershifted band was observed with 0.3 µg of anti-p65 antibody (arrowhead). Lane 1 = WT with EMCV inoculation; lane 2 = lane 1 with a 100-fold excess of cold probe; lane 3 = lane 1 with anti-p65 antibody; lane 4 = without nuclear protein (negative control). (B) Representative EMSA experiment. From each sample, equal amounts of nuclear protein were obtained and mixed in each group (n = 8 each). The experiments were performed using 8 µg of the mixed proteins. Lane 1 = WT with phosphate buffered saline (PBS) inoculation; lane 2 = knock-out (KO) with PBS inoculation; lane 3 = wild-type (WT) with EMCV inoculation; lane 4 = KO with EMCV inoculation. (C) Densitometric analysis of the results expressed as arbitrary units of mean values ± SEM of eight animals from each group. The mean value of nuclear factor-kappa B activities in the WT group with PBS inoculation is represented as 1. *p < 0.01 vs. WT with PBS inoculation. #p < 0.01 vs. KO with EMCV inoculation.
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