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Figure 5 Effect of bovine anti-LOX-1 monoclonal antibody (bLOX-1 mAb), nebivolol, and its 4-keto derivative alone or in combination with bLOX-1 mAb on oxidized low-density lipoprotein (ox-LDL)-induced variations of nitric oxide (NO) in bradykinin-stimulated bovine aortic endothelial cells (BAECs). Nebivolol (25 µmol/l), its 4-keto derivative (25 µmol/l), and bLOX-1 mAb (30 µg/ml) were preincubated with BAECs for 30 min at 37°C. The cells were then stimulated with ox-LDL (50 µg protein/ml) for 10 min at 37°C. Results are expressed as mean fluorescence intensity (MFI) and are means ± SD of experiments performed in triplicate on six separate occasions. *p < 0.001 versus control;  p < 0.001 versus ox-LDL; ¶p < 0.01 versus bLOX-1 mAb.
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