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Figure 1 Effect of native low-density lipoprotein (LDL), oxidized LDL (ox-LDL), and ox-LDL plus anti-human LOX-1 monoclonal antibody (hLOX-1 mAb), SB203580 and bisindolylmaleimide on the translocation of p47phox and Rac1 to the plasma membrane of human umbilical vein endothelial cells. The cells were preincubated with hLOX-1 mAb, SB203580, and bisindolylmaleimide for 30 min at 37°C and then stimulated with native LDL (control) and ox-LDL (50 µg protein/ml) for 5 min at 37°C. The figure shows representative blots of six different experiments and the average data obtained by densitometric analysis. Results (means ± SD) are expressed as density in arbitrary units (AU). *p < 0.001 versus control; ¶p < 0.001 versus ox-LDL;  p < 0.05 versus ox-LDL. Solid bars = p47phox; open bars = Rac1.
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