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Figure 5 (A) The reverse transcription-polymerase chain reaction technique demonstrates that freshly isolated human adipocytes and non-adipocyte adipose stromal cells (ASCs) express hepatocyte growth factor (HGF). The expression appears to be less than that of liver tissue, when comparable amounts of total deoxyribonucleic acid (see glyceraldehyde-3-phosphate dehydrogenase as control) are used. (B) Adipocytes and ASCs can also secrete HGF protein into conditioned media, and achieve biologically active HGF concentrations in the media, which are substantially higher than those of cultured human aortic endothelial cells (HAECs).
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