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Figure 2 Representative transgene expression demonstrated by reverse-transcription nested polymerase chain reaction (RT-nested PCR). The left adductor muscle of the rabbits was injected with naked fibroblast growth factor 4 (FGF4) gene (lanes 1 to 3), gelatin hydrogel (GHG)-FGF4 (lanes 4 to 6), or GHG-lacZ (lanes 7 and 8). Each sample was obtained from the injection site (lanes 1, 4, and 7) and the adjacent region 10 mm apart from the injection site (lanes 2, 5 and 8) in the left adductor muscle, and from the contralateral adductor muscle (lanes 3 and 6). The RT-nested PCR products from ribonucleic acid of each sample were analyzed on agarose gel; FGF4 expressed Cc1/l6 cells as a positive control (lane 9) and no deoxyribonucleic acid (DNA) template as a negative control (lane 10). A housekeeping beta-actin gene was amplified as a complementary DNA loading control.
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