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Figure 1 Expression of lectin-like oxidized low-density lipoprotein receptor (LOX-1) messenger ribonucleic acid (mRNA) and protein after ischemia-reperfusion (I-R); LOX-1 protein and mRNA were determined by Western and Northern blots, respectively. The expression of LOX-1 was markedly increased in the saline-treated rats subjected to I-R. Ischemia alone did not increase the expression of LOX-1. Administration of blocking antibody to rat LOX-1 attenuated the expression of LOX-1 despite I-R. In contrast, administration of nonspecific immunoglobulin G (IgG) had no effect. Density of LOX-1 mRNA band was normalized by glyceraldehyde-3-phosphate dehydrogenase (GAPDH) band density. Each band density of LOX-1 protein was normalized with that in the sham control rat heart. Left panel shows representative examples; right panel is the summary of data (mean ± SD) from eight separate experiments.
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