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Figure 3 Effects of rapid atrial pacing on the expression of Ca²⁺ channel ₁, ₂, CaB2a, CaB2b and CaB3 subunits. (A) Representative agarose gels for semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) of the rabbit calcium channel genes ₁ and ₂, and G3PDH as standard. (B) Representative gels of Western blots showing a specific band at 190 kD. Expression was normalized to that of G3PDH. (C) Histogram of the densitometric analysis for ₁ (messenger ribonucleic acid [mRNA] + protein) and ₂ (mRNA). (D) Representative agarose gels for semiquantitative RT-PCR of the rabbit calcium channel genes CaB2a, CaB2b, and CaB3. (E) Histogram of the densitometric analysis. *p < 0.05 and **p < 0.01 vs. P0 (One-way analysis of variance. Comparisons between multiple groups were performed with a Bonferroni corrected t test).

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