Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 1 Characterization of apoptotic myocardiocytes. (A) Hematoxylin/van Gieson stain. Site of recent infarction: reparative fibrosis, newly sprouted vessels, and granulation tissue (original magnification x500). (B) In situ end labeling of deoxyribonucleic acid (TUNEL) staining. Region of the left ventricle at the site of infarction: TUNEL-positive nuclei (brown) are evident (original magnification x500; 3-amino-9-ethylcarbazide [AEC], lightly counterstained with hematoxylin). (C) Double staining: nuclear staining for TUNEL and cytoplasmic staining for muscle actin. TUNEL-positive cells (brown nuclear staining) co-express cytoplasmic muscle actin (original magnification x600; AEC, lightly counterstained with hematoxylin). (D) Double staining: nuclear staining for TUNEL and cytoplasmic staining for caspase-3 (original magnification x600; AEC, lightly counterstained with hematoxylin).
|
