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Figure 7 Functional experiments of the mutated gene product. Whole-cell patch-clamp recordings of wild-type SCN5A (A and B) and  exon 22-SCN5A (C and D) in the absence (A and C) or presence (B and D) of the beta-1 regulatory subunit. Holding potential: –100 mV. Depolarizing steps from –60 mV to +30 mV in 10 mV increments. Vertical bar = 0.5 nA. Horizontal bar = 5 ms. (E) Membrane trafficking of GFP-tagged wild-type (WT) or exon 22 deleted (exon 22) proteins. Top panels = conventional microscopy; lower panels = confocal microscopy imaging. Scaling is 10 microns.
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