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Figure 1 Ethidium bromide gel showing three genotypes from a single nucleotide polymorphism (C or T) in the promoter region 129 bases upstream of the GCLC gene. The 613-bp polymerase chain reaction amplification fragment contains an invariant Tsp45I restriction site, yielding a constant 113-bp fragment seen in all lanes. Individuals homozygous for the C allele have no additional Tsp45I restriction sites and consequently show only two bands: at 500-bp and the invariant band at 113-bp. The –129T allele creates an additional Tsp45I site such that homozygous T-allele individuals have three bands at 302-, 198-, and 113-bp. Heterozygous individuals show all four bands.
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