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Figure 3 Effect of anti-LOX-1 monoclonal antibody (LOX-1 Ab), anti-CD41a antibody, annexin V, and immunoglobulin G (IgG) on platelet-induced variations of superoxide concentration in bovine aortic endothelial cells (BAECs). The LOX-1 Ab (30 µg/ml), anti-CD41a antibody (30 µg/ml) alone and mixed with LOX-1 Ab, annexin V (10 µmol/l), and IgG (30 µg/ml) were preincubated with BAECs for 30 min at 37°C. Thrombin-activated platelets (3 x 107/ml) were added to the cell medium for 10 min at 37°C. Results are expressed as percent variations of mean fluorescence intensity (MFI) and are the means ± SD of experiments performed in triplicate on six separate occasions. *p < 0.001 vs. platelets alone.
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