Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 1 Effect of red wines on endothelial-type nitric oxide synthase (eNOS) messenger ribonucleic acid (mRNA) expression in human endothelial EA.hy 926 cells. Cells were left untreated (control, Co) or exposed for 24 h to ethanol (EtOH-Co, 1.25%, v/v), three different German red wines ("Dornfelder", "Kriegsheimer Rosengarten", and "Spätburgunder") or six different French red wines from Bordeaux ("Ch.Bonnet reserve", "Ch.Bellegrave", and "Ch.Marechaude"), Rhone ("Ch.Cabieres"), and Burgundy ("Ch.Rousseau", "L.Chevaliers"). The red wines were diluted in the cell culture medium to a final concentration of 10% (v/v). RNase protection analyses were performed using antisense RNA probes to human eNOS and GAPDH (for standardization). The figure shows densitometric analyses of three different gels. Bars represent means ± SEM. Asterisks indicate significant differences from untreated cells (***p < 0.001; by ANOVA followed by the Fisher PLSD test).
|
