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Figure 5 (A) Deoxyribonucleic acid (DNA)-binding activity of nuclear factor (NF)-kappa-B measured by EMSA with supershift in the right ventricular myocardium of infants with tetralogy of Fallot (TOF) (solid bar) or ventricular septal defect (VSD) (open bar). Results are expressed as counts (CNT)/mm2. (B) Exemplary gel showing the presence NF-kappa-B (arrow) detected by electrophoretic mobility shift assay and supershift with anti-p65 and anti-p50 in nuclear extracts of the right ventricular myocardium of one patient with TOF and one patient with VSD. This gel is representative of those obtained in three patients from each group. PC = positive control (human HepG2 cells stimulated by TNF-alpha); NS = nonspecific.
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