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EDITORIAL COMMENT

Genomic medicine and atrial fibrillation^*

^* University of Colorado Cardiovascular Institute and Adult Medical Genetic Program, University of Colorado Health Sciences Center, Denver, Colorado, USA

^* Reprint requests and correspondence: Dr. Luisa Mestroni, CUCVI and AMGP, Bioscience Park Center, Suite 150, 12635 E. Montview Blvd., Aurora, Colorado 80010-7116, USA.
Luisa.Mestroni{at}uchsc.edu

	Epidemiology and clinical implications of atrial fibrillation

Top Epidemiology and clinical... Genetic epidemiology of AF Role of gene mutations... Role of the changes... Conclusions References

 
Atrial fibrillation (AF) is a common disorder that is proving to be an excellent example of the interaction between environmental and genetic factors. Atrial fibrillation is one of the primary causes of mortality, morbidity, and socioeconomic consequences from hospitalizations. Atrial fibrillation is characterized by a rapid and irregular activation of the atrial myocardium at a rate of approximately 400 to 600 pulses/min. The uncontrolled rapid ventricular response rate may eventually cause ventricular dysfunction and heart failure. Additionally, the loss of effective atrial contraction may also cause blood stasis and promote the occurrence of thromboemboli and stroke. As a result, AF requires long-term anticoagulant and rate-control therapy, and sometimes more sophisticated treatments, such as ablation and implantable devices.

Atrial fibrillation and atrial flutter account for approximately 9,000 deaths and 400,000 hospitalizations annually, and they are the cause of stroke in about 15% of patients (2). The prevalence of AF is estimated to be between 0.5 and 1% in the general population, and the condition affects approximately 2.2 million individuals in the U.S. alone (3). The incidence and prevalence of AF increases with age: approximately 70% of those diagnosed with AF are between 65 and 85 years old (3). Braunwald (4) has recently described AF as a growing "epidemic" as the population of elderly continues to increase. Still, the real frequency of AF is probably underestimated (5) because of the high rate (30% to 45%) of asymptomatic arrhythmia, which frequently goes undetected.

In spite of AF’s clinical relevance, the origin and the mechanisms underlying this common arrhythmia have, until recently, been obscure. Atrial fibrillation has been recognized since the early 1900s and typically has been associated with conditions that increase atrial pressure or cause atrial dilation (Table 1). Atrial fibrillation is also associated with cardiomyopathies (6–10), diseases of the cardiac muscle which are known to be genetically determined in a large proportion of patients. Finally, AF may also occur in patients without any other evidence of heart or systemic disease, a condition known as lone AF. Lone AF accounts for 2% to 31% of the AF patient population (5) and usually affects young and middle-aged adults.

	Genetic epidemiology of AF

Top Epidemiology and clinical... Genetic epidemiology of AF Role of gene mutations... Role of the changes... Conclusions References

 
The familial occurrence of AF has been known for many decades yet traditionally has been considered a rare event (5,11). However, the study of the familial forms of AF caused by a gene defect was considered key to the discovery of factors that constitute initiation of AF.

The first important advance in this direction has been the identification of a genetic locus for familial AF on chromosome 10q22-q24, by Brugada et al. (12). Three families from Catalonia, Spain, were studied and showed an autosomal dominant pattern of transmission and early onset of the arrhythmia (age 1 to 45 years). Positional cloning and analysis of candidate genes to identify the disease gene are currently ongoing. Since the beginning of their studies, Brugada et al. (11) have collected probands from more than 100 families with familial AF. Their findings revealed that not all families were affected by the chromosome 10q22-q24 locus, suggesting that familial AF is a genetically heterogeneous disorder caused by more than one gene. Their findings also supported the notion that familial AF may be more common than previously suspected.

In this issue of the Journal, Darbar et al. (13) report the results of a large survey performed in the arrhythmia clinic at the Mayo Clinic in Rochester, Minnesota. Out of 914 patients with AF, 36% had lone AF, and of that patient population, 15% had a familial history of the disorder (5% of the overall population). These findings discount the old idea that familial AF is a rare condition. The age of onset in the 50 probands with familial AF ranged from 25 to 55 years.

The investigators were able to identify four multigeneration families that showed an autosomal dominant pattern of transmission, as previously reported. Interestingly, none of them showed linkage with the locus on chromosome 10 that was reported in the families from Catalonia, suggesting the existence of yet another gene or multiple genes.

The other intriguing feature was that different ventricular responses and different outcomes were observed in the phenotype analysis. In three families, the affected family members had symptomatic AF with rapid ventricular response, in some instances leading to tachycardia-induced cardiomyopathy, which improved after sinus rhythm restoration or rate-control. These families had also experienced a significant incidence of stroke. On the other hand, affected members of the fourth family frequently had asymptomatic AF, with slow ventricular response, atrial myopathy, progression to conduction delay, and "ventricular" cardiomyopathy.

The two different phenotypes described in the study of Darbar et al. (13) may indicate two distinct mechanisms (and corresponding genes or pathways) involved in the origin of AF. The phenotype with AF and slow ventricular response suggests an underlying more diffuse myocardial disease. Indeed, the coexistence of atrial arrhythmia (including AF) and conduction defects are found in cardiomyopathies caused by mutations of cytoskeletal genes of the nuclear lamina, such as emerin and lamin (Table 1) (6,14). On the other hand, the phenotype with AF rapid ventricular response and without primary involvement of the "working" myocardium may suggest an ion channel disease, such as cases of ventricular arrhythmia due to sodium channel mutations (long QT syndrome, Brugada syndrome, or idiopathic ventricular tachycardia) (14). Most recent advances in the molecular genetics of AF support this hypothesis and are discussed later.

The attempt to define the epidemiology of familial AF by Darbar et al. (13) has several limitations. These include the possibility of a referral bias overestimating the frequency of lone AF, an underestimate due to clinically silent forms, or survey results based on family history rather than clinical evaluation of relatives. Further, the relative frequency of the rapid- and slow-ventricular rate AF in the overall population is unknown. However, the relevant messages emerging from this study are that the familial occurrence of AF is not rare, that the condition is probably due to several different genes and molecular mechanisms, that it may be associated with young age and complications such as stroke and cardiomyopathy, and that patients with this disorder should undergo adequate screening, therapy, and counseling.

	Role of gene mutations in the origin of AF

Top Epidemiology and clinical... Genetic epidemiology of AF Role of gene mutations... Role of the changes... Conclusions References

 
The hypothesis that ion channels could have a major role in causing AF has been recently confirmed by two studies. The first study tested the hypothesis of an association between AF and a genetic variation (polymorphism) in the minK gene (KCNE1), encoding the beta subunit of the I_Ks potassium channel (KvLQT1/minK) (15). The I_Ks potassium channel, formed by the alpha (KvLQT1) and beta (minK) subunits, determines the "slow" component of the K⁺ current, causing cellular depolarization in atrial myocardium. In a Chinese population with risk factors for AF (including valvular heart disease, hypertension, and left ventricular dysfunction), patients with the 38G allele were more likely to develop AF than patients with the 38S allele. Unfortunately, the functional significance and the electrophysiologic consequences of the genetic variation are currently unknown. However, these findings may provide new insights into the pathophysiology and potential treatment of the disease.

The second recent study reports the discovery of the first gene causing lone AF in a Chinese family with autosomal dominant transmission (16). Not surprisingly, the disease-gene (KCNQ1 or KvLQT1) encodes the alpha subunit of the cardiac I_Ks potassium channel, as well as the KCNQ1/KCNE2 and KCNQ1/KCNE3 potassium channels, and is again involved in atrial repolarization. The disease-causing mutation (S140G) causes a gain-of-function of the KCNQ1/KCNE1 and KCNQ1/KCNE2 channels, reducing the action potential duration and the effective refractory period, therefore inducing a mechanism able to initiate and maintain AF. Interestingly, different mutations in the same gene were found to cause loss-of-function and lead to long QT syndrome (17). The phenotypic heterogeneity of KCNQ1 is not surprising, as it is also observed in other ion channel diseases. For example, as mentioned before, different mutations in SCN5A may cause long QT, conduction defects, and Brugada syndrome (14). The finding of major involvement of K⁺ channels in the etiology of AF offers the opportunity to develop novel therapeutic strategies, such as I_Ks blockers.

	Role of the changes in gene expression (electrical remodeling) in maintaining AF

Top Epidemiology and clinical... Genetic epidemiology of AF Role of gene mutations... Role of the changes... Conclusions References

 
A well-known principle in human genetics states that every phenotype is determined by the interaction of three factors: major genes with a strong effect; several weak genes with a modifier effect, leading to susceptibility or protection; and the environment. In the case of AF phenotype, recent studies have shown that changes in gene expression (the molecular environment) are critical in maintaining the arrhythmia and transforming paroxysmal episodes in a chronic irreversible condition. Once initiated, AF induces an alteration of the electrophysiological properties that favors induction and maintenance of the arrhythmia—a process called "electrical remodeling" (18). The rapid atrial rate causes a progressive intracellular Ca²⁺ loading, which threatens cell viability and induces a final common pathway of defensive responses in the cell. These include transcriptional down-regulation of the Ca²⁺ channel (I_Ca), decrease of the Ca²⁺ current, alteration in both the inward and outward K⁺ currents, and decrease of the action potential and of the refractory period that eventually promotes and maintains AF by multiple-circuit re-entry. Furthermore, the Ca²⁺ overload and the decreased Ca²⁺ exchange induce cellular myolysis and activation of the fetal gene program leading to structural remodeling, and reduce atrial contraction (atrial stunning) contributing to blood stasis and thromboembolism. Overall, the atrial ionic remodeling follows pathways very similar to those in ventricular remodeling in heart failure, suggesting a common underlying mechanism (19).

The advances in understanding the remodeling process may have important clinical implications in terms of prevention (such as early cardioversion, implantable atrial defibrillator, and preventive atrial pacing methods) and therapies to drive reverse remodeling (5). This new understanding will clearly influence future investigations of genes involved in the remodeling pathways that may cause or modulate AF.

	Conclusions

Top Epidemiology and clinical... Genetic epidemiology of AF Role of gene mutations... Role of the changes... Conclusions References

 
The new insights on genes and genotype/phenotype correlations involved in AF have impressively increased our understanding of the pathophysiological mechanisms leading to arrhythmia. The new knowledge supports investigations about novel disease genes, their interactions with remodeling mechanisms, and potential therapies to treat AF at the ion channel and cellular levels. In this process, unraveling the clinical and molecular genetics of AF has a central role that may provide new insights into the structural and electrical phenotypes resulting from genetic mutations. Eventually genomic information in AF patients will impact the optimal approach to care, from prevention and diagnosis to therapy and counseling. At that point systematic genetic screening will become standard in the management of familial AF in clinical practice.

	Acknowledgments

 
Michael R. Bristow, MD, PhD, UCHSC, is gratefully acknowledged for his critical reading and helpful suggestions. Laurel Hunter is also gratefully acknowledged for her help in manuscript review and editing.

	Footnotes

 
Dr. Mestroni receives support from the National Institutes of Health/National Heart, Lung, and Blood Institute (1RO1 HL69071-01), the Muscular Dystrophy Association U.S.A. (PN0007056), and the American Heart Association (0250271N).

^* Editorials published in the Journal of the American College of Cardiology reflect the views of the authors and do not necessarily represent the views of JACC or the American College of Cardiology.

	References

Top Epidemiology and clinical... Genetic epidemiology of AF Role of gene mutations... Role of the changes... Conclusions References

 

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