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Figure 5 Cardiomyocyte apoptosis in remote areas from infarction observed under fluorescent light. Panels A, B, and F depict representative triple staining obtained in the same microscopic fields from a growth hormone (GH)-treated rat euthanized at one month. Panel A shows cardiomyocyte and non-cardiomyocyte nuclei stained with DAPI. Panel B depicts cardiomyocyte immunostained with anti-sarcomeric actin. Panels C to F depict detection of terminal UTP nick end labeling (TUNEL)-positive cardiomyocyte nuclei. Panels C and D are representative images from placebo and GH group, respectively, after the 13-month observation period. Panels E and F are representative images from placebo and GH rats, respectively, euthanized at one month. Note the higher percentage of positive nuclei in the placebo-treated animals. Panel G: Immunostaining for activated caspase-3 antibody. Panel H: Double-exposure photomicrograph of dual labeled cardiomyocytes for activated caspase-3 (red signal) and TUNEL. Note the co-localization of caspase activity and TUNEL-positivity in the same cardiomyocyte. Overlapped signals result in yellow fluorescence. Bars = 20 µm.
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